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Olympus laser scanning confocal microscope head fv3000
A) To optimise the seeding of MDMs and HUVECs into a Matrigel model, cells were first both mixed with Matrigel, resulting in no tube formation or cell association. B) MDMs were mixed in Matrigel and HUVECs were seeded on top, resulting in tube formation, but no cell association. C) Matrigel was plated first and both cell types were added on top, resulting in tube formation and cell co-localisation. D) Representative images of all individual channels of the complete model. Green = HUVECs, magenta = MDMs, and blue = nuclear stain. White indicates the overlapping of colours. Images were recorded on a Confocal <t>FV3000</t> Laser Scanning Microscope (Olympus) and Z projections were created using Fiji. Scale bar represents 200 μm (A-C) or 500 μm (D).
Laser Scanning Confocal Microscope Head Fv3000, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+laser+scanning+head+system/FV3000+Confocal+Laser+Scanning+Microscope/bio_rxiv__2025__07__16__665214-85-12-18
Average 99 stars, based on 1 article reviews
laser scanning confocal microscope head fv3000 - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

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A) To optimise the seeding of MDMs and HUVECs into a Matrigel model, cells were first both mixed with Matrigel, resulting in no tube formation or cell association. B) MDMs were mixed in Matrigel and HUVECs were seeded on top, resulting in tube formation, but no cell association. C) Matrigel was plated first and both cell types were added on top, resulting in tube formation and cell co-localisation. D) Representative images of all individual channels of the complete model. Green = HUVECs, magenta = MDMs, and blue = nuclear stain. White indicates the overlapping of colours. Images were recorded on a Confocal FV3000 Laser Scanning Microscope (Olympus) and Z projections were created using Fiji. Scale bar represents 200 μm (A-C) or 500 μm (D).

Journal: bioRxiv

Article Title: Development of a 3D in vitro wound healing model to assess the effect of ADSC-EVs on vascularisation

doi: 10.1101/2025.07.16.665214

Figure Lengend Snippet: A) To optimise the seeding of MDMs and HUVECs into a Matrigel model, cells were first both mixed with Matrigel, resulting in no tube formation or cell association. B) MDMs were mixed in Matrigel and HUVECs were seeded on top, resulting in tube formation, but no cell association. C) Matrigel was plated first and both cell types were added on top, resulting in tube formation and cell co-localisation. D) Representative images of all individual channels of the complete model. Green = HUVECs, magenta = MDMs, and blue = nuclear stain. White indicates the overlapping of colours. Images were recorded on a Confocal FV3000 Laser Scanning Microscope (Olympus) and Z projections were created using Fiji. Scale bar represents 200 μm (A-C) or 500 μm (D).

Article Snippet: All cultures were visualised and imaged on inverted microscope IX83 equipped with Laser Scanning Confocal Microscope head FV3000 (Olympus, Japan) using software FV31-SW, version 2.4198.

Techniques: Staining, Laser-Scanning Microscopy